› 2lfj

Solution NMR

Solution structure of the monomeric derivative of BS-RNase

Released:
DOI: 10.2210/pdb2lfj/pdb
PDB entry 2lfj coloured by chain, ensemble of 10 models, front view.
PDB entry 2lfj coloured by chain, ensemble of 10 models, side view.
PDB entry 2lfj coloured by chain, ensemble of 10 models, top view.
Source organism: Bos taurus
Primary publication:
NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping.
Spadaccini R, Ercole C, Gentile MA, Sanfelice D, Boelens R, Wechselberger R, Batta G, Bernini A, Niccolai N, Picone D
PLoS One 7 e29076 (2012)
PMID: 22253705

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seminal ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.63 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00669 (Residues: 27-150; Coverage: 100%)
Gene name: SRN
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 71%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural and functional importance of local and global conformational fluctuations in the RNase A superfamily.
Gagné et al. (2013)
1 more

1 mention without citation

NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping.
Spadaccini et al. (2012)