› 2lkd

Solution NMR

IF2-G2 GDP complex

Released:
DOI: 10.2210/pdb2lkd/pdb
PDB entry 2lkd coloured by chain, ensemble of 18 models, front view.
PDB entry 2lkd coloured by chain, ensemble of 18 models, side view.
PDB entry 2lkd coloured by chain, ensemble of 18 models, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structural dynamics of bacterial translation initiation factor IF2.
Wienk H, Tishchenko E, Belardinelli R, Tomaselli S, Dongre R, Spurio R, Folkers GE, Gualerzi CO, Boelens R
J Biol Chem 287 10922-32 (2012)
PMID: 22308033

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translation initiation factor IF-2 Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 19.52 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04766 (Residues: 241-414; Coverage: 24%)
Gene name: infB
Sequence domains: Elongation factor Tu GTP binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Initiation of mRNA translation in bacteria: structural and dynamic aspects.
Gualerzi et al. (2015)
3 more

3 mentions without citation

Initiation factor 2 crystal structure reveals a different domain organization from eukaryotic initiation factor 5B and mechanism among translational GTPases.
Eiler et al. (2013)
2 more