› 2lkf

X-ray diffraction
2.5Å resolution

LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS

Released:
DOI: 10.2210/pdb2lkf/pdb
PDB entry 2lkf coloured by chain, front view.
PDB entry 2lkf coloured by chain, side view.
PDB entry 2lkf coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel.
Olson R, Nariya H, Yokota K, Kamio Y, Gouaux E
Nat Struct Biol 6 134-40 (1999)
PMID: 10048924

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-hemolysin component B Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.09 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A077 (Residues: 27-325; Coverage: 100%)
Gene name: hlgB
Sequence domains: Leukocidin/Hemolysin toxin family
Structure domains: Leukocidin/porin MspA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 49.22Å b: 49.52Å c: 127.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.323
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
18 more

1 mention without citation

Structural and functional analysis of the pore-forming toxin NetB from Clostridium perfringens.
Yan et al. (2013)