› 2lml

Solution NMR

Solution NMR structure of holo acyl carrier protein from geobacter Metallireducens refined with nh rdcs, Northeast Structural Genomics consortium target gmr141

Released:
DOI: 10.2210/pdb2lml/pdb
PDB entry 2lml coloured by chain, ensemble of 20 models, front view.
PDB entry 2lml coloured by chain, ensemble of 20 models, side view.
PDB entry 2lml coloured by chain, ensemble of 20 models, top view.
Source organism: Geobacter metallireducens GS-15
Primary publication:
Solution structure of 4'-phosphopantetheine - GmACP3 from Geobacter metallireducens: a specialized acyl carrier protein with atypical structural features and a putative role in lipopolysaccharide biosynthesis.
Ramelot TA, Smola MJ, Lee HW, Ciccosanti C, Hamilton K, Acton TB, Xiao R, Everett JK, Prestegard JH, Montelione GT, Kennedy MA
Biochemistry 50 1442-53 (2011)
PMID: 21235239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carrier domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.02 KDa
Source organism: Geobacter metallireducens GS-15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39T60 (Residues: 1-79; Coverage: 100%)
Gene names: Gmet_2339, acpP-4
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments

1 bound ligand:
Ligand PNS 1 x PNS
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The structural role of the carrier protein--active controller or passive carrier.
Crosby et al. (2012)
2 other articles

1 mention without citation

Probing the phosphopantetheine arm conformations of acyl carrier proteins using vibrational spectroscopy.
Johnson et al. (2014)