› 2ln3

Solution NMR

Solution NMR Structure of DE NOVO DESIGNED PROTEIN, IF3-like fold, Northeast Structural Genomics Consortium Target OR135 (CASD target)

Released:
DOI: 10.2210/pdb2ln3/pdb
PDB entry 2ln3 coloured by chain, ensemble of 20 models, front view.
PDB entry 2ln3 coloured by chain, ensemble of 20 models, side view.
PDB entry 2ln3 coloured by chain, ensemble of 20 models, top view.
Source organism: synthetic construct
Primary publication:
Principles for designing ideal protein structures.
Koga N, Tatsumi-Koga R, Liu G, Xiao R, Acton TB, Montelione GT, Baker D
Nature 491 222-7 (2012)
PMID: 23135467

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DE NOVO DESIGNED PROTEIN OR135 Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.8 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Translation Initiation Factor IF3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 93%
Refinement method: simulated annealing, molecular dynamics, distance geometry, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

60 review citations

The coming of age of de novo protein design.
Huang et al. (2016)
59 more

11 mentions without citation

Principles for designing ideal protein structures.
Koga et al. (2012)
10 more