Function and Biology Details
Reaction catalysed:
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
NEDD8-activating enzyme E1 catalytic subunit (preferred)
PDBe Complex ID:
PDB-CPX-186174 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NEDD8-activating enzyme E1 catalytic subunit
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: E2 binding domain
Structure domains: Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3
Length: 97 amino acids
Theoretical weight: 10.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q8TBC4 (Residues: 369-463; Coverage: 21%)
Sequence domains: E2 binding domain
Structure domains: Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
90%
Refinement method:
torsion angle dynamics
Expression system: Escherichia coli
