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Solution NMR

Solution Structures of the IIA(Chitobiose)-HPr complex of the N,N'-Diacetylchitobiose Branch of the Escherichia coli Phosphotransferase System

Released:
DOI: 10.2210/pdb2lrl/pdb
PDB entry 2lrl coloured by chain, ensemble of 20 models, front view.
PDB entry 2lrl coloured by chain, ensemble of 20 models, side view.
PDB entry 2lrl coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli K-12
Primary publication:
Solution structure of the IIAChitobiose-HPr complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.
Jung YS, Cai M, Clore GM
J Biol Chem 287 23819-29 (2012)
PMID: 22593574

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141946 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system N,N'-diacetylchitobiose-specific EIIA component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 103 amino acids
Theoretical weight: 11.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69791 (Residues: 14-116; Coverage: 89%)
Gene names: JW1725, b1736, celC, chbA
Sequence domains: PTS system, Lactose/Cellobiose specific IIA subunit
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
Phosphocarrier protein HPr Chain: D
Molecule details ›
Chain: D
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Gene names: JW2408, b2415, hpr, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

1 bound ligand:
Ligand PO3 1 x PO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 37%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure, dynamics and biophysics of the cytoplasmic protein-protein complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system.
Clore et al. (2013)
5 other articles

2 mentions without citation

Solution structure of the IIAChitobiose-HPr complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.
Jung et al. (2012)
1 more