Function and Biology Details
Reaction catalysed:
Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
Peptidase, M23/M37 family (preferred)
PDBe Complex ID:
PDB-CPX-129493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase, M23/M37 family
Molecule details ›
Chain: 1
Length: 128 amino acids
Theoretical weight: 14.09 KDa
Source organism: Streptococcus equi subsp. zooepidemicus
Expression system: Escherichia coli
UniProt:
Sequence domains: Target recognition domain of lytic exoenzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Length: 128 amino acids
Theoretical weight: 14.09 KDa
Source organism: Streptococcus equi subsp. zooepidemicus
Expression system: Escherichia coli
UniProt:
- Canonical:
O54308 (Residues: 170-285; Coverage: 44%)
Sequence domains: Target recognition domain of lytic exoenzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
91%
Refinement method:
simulated annealing, molecular dynamics
Expression system: Escherichia coli
