Function and Biology Details
Reaction catalysed:
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domain:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase AMFR
Molecule details ›
Chain: C
Length: 52 amino acids
Theoretical weight: 5.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: CUE domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain
Length: 52 amino acids
Theoretical weight: 5.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q9UKV5 (Residues: 453-504; Coverage: 8%)
Sequence domains: CUE domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
90%
Refinement method:
simulated annealing
Expression system: Escherichia coli
