› 2lxt

Solution NMR

Allosteric communication in the KIX domain proceeds through dynamic re-packing of the hydrophobic core

Released:
DOI: 10.2210/pdb2lxt/pdb
PDB entry 2lxt coloured by chain, ensemble of 20 models, front view.
PDB entry 2lxt coloured by chain, ensemble of 20 models, side view.
PDB entry 2lxt coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric communication in the KIX domain proceeds through dynamic repacking of the hydrophobic core.
Brüschweiler S, Konrat R, Tollinger M
ACS Chem Biol 8 1600-10 (2013)
PMID: 23651431

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-147661 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CREB-binding protein Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92793 (Residues: 587-673; Coverage: 4%)
Gene names: CBP, CREBBP
Sequence domains: KIX domain
Structure domains: Coactivator CBP, KIX domain
MLL cleavage product C180 Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03164 (Residues: 2840-2858; Coverage: 1%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
Cyclic AMP-responsive element-binding protein 1 Chain: C
Molecule details ›
Chain: C
Length: 34 amino acids
Theoretical weight: 3.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16220 (Residues: 102-135; Coverage: 10%)
Gene name: CREB1
Sequence domains: pKID domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 50%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Role of Intrinsic Protein Disorder in the Function and Interactions of the Transcriptional Coactivators CREB-binding Protein (CBP) and p300.
Dyson et al. (2016)
4 more

24 mentions without citation

Molecular recognition by the KIX domain and its role in gene regulation.
Thakur et al. (2014)
23 more