› 2m2w

Solution NMR

Ternary complex of ASFV Pol X with DNA and MgdGTP

Released:
DOI: 10.2210/pdb2m2w/pdb
PDB entry 2m2w coloured by chain, ensemble of 10 models, front view.
PDB entry 2m2w coloured by chain, ensemble of 10 models, side view.
PDB entry 2m2w coloured by chain, ensemble of 10 models, top view.
Source organism: African swine fever virus
Primary publication:
How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation.
Wu WJ, Su MI, Wu JL, Kumar S, Lim LH, Wang CW, Nelissen FH, Chen MC, Doreleijers JF, Wijmenga SS, Tsai MD
J Am Chem Soc 136 4927-37 (2014)
PMID: 24617852

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115583 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Repair DNA polymerase X Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.35 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P42494 (Residues: 1-174; Coverage: 100%)
Gene names: Ba71V-97, O174L
Sequence domains:
Structure domains:
5'-D(P*GP*GP*CP*GP*AP*AP*GP*CP*CP*GP*GP*GP*TP*GP*CP*GP*AP*AP*GP*CP*AP*CP*(DOC))-3' Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.13 KDa
Source organism: African swine fever virus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand DGT 1 x DGT
Ligand MG 2 x MG
1 modified residue:
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 37%
Refinement method: distance geometry
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Stable isotope labeling methods for DNA.
Nelissen et al. (2016)
11 other articles