› 2m3g

Solid-state NMR

Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy

Released:
DOI: 10.2210/pdb2m3g/pdb
PDB entry 2m3g coloured by chain, ensemble of 10 models, front view.
PDB entry 2m3g coloured by chain, ensemble of 10 models, side view.
PDB entry 2m3g coloured by chain, ensemble of 10 models, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein.
Wang S, Munro RA, Shi L, Kawamura I, Okitsu T, Wada A, Kim SY, Jung KH, Brown LS, Ladizhansky V
Nat Methods 10 1007-12 (2013)
PMID: 24013819

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 235 amino acids
Theoretical weight: 27.24 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8YSC4 (Residues: 1-229; Coverage: 88%)
Gene name: alr3165
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 3 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 12%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21

Quick links

Citations

21 review citations

Computational modeling of membrane proteins.
Koehler Leman et al. (2015)
20 more

7 mentions without citation

Structure and Dynamics of Membrane Proteins from Solid-State NMR.
Mandala et al. (2018)
6 more