› 2m4n

Solution NMR

Solution structure of the putative Ras interaction domain of AFD-1, isoform a from Caenorhabditis elegans

Released:
DOI: 10.2210/pdb2m4n/pdb
PDB entry 2m4n coloured by chain, ensemble of 20 models, front view.
PDB entry 2m4n coloured by chain, ensemble of 20 models, side view.
PDB entry 2m4n coloured by chain, ensemble of 20 models, top view.
Source organism: Caenorhabditis elegans
Entry authors: Harris R, Hillerich B, Ahmed M, Bonanno JB, Chamala S, Evans B, Lafleur J, Hammonds J, Washington E, Stead M, Love J, Attonito J, Seidel RD, Liddington RC, Weis WI, Nelson WJ, Girvin ME, Almo SC, New York Structural Genomics Research Consortium (NYSGRC), Assembly Dynamics and Evolution of Cell-Cell and Cell-Matrix Adhesions (CELLMAT)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Afadin Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 11.97 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BIC1 (Residues: 214-320; Coverage: 7%)
Gene names: CELE_W03F11.6, W03F11.6, afd-1
Sequence domains: Ras association (RalGDS/AF-6) domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: simulating annealing
Expression system: Escherichia coli BL21(DE3)

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