› 2m5a

Solution NMR

Protein A binding by an engineered Affibody molecule

Released:
DOI: 10.2210/pdb2m5a/pdb
PDB entry 2m5a coloured by chain, ensemble of 20 models, front view.
PDB entry 2m5a coloured by chain, ensemble of 20 models, side view.
PDB entry 2m5a coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
High-affinity binding to staphylococcal protein A by an engineered dimeric Affibody molecule.
Lindborg M, Dubnovitsky A, Olesen K, Björkman T, Abrahmsén L, Feldwisch J, Härd T
Protein Eng Des Sel 26 635-44 (2013)
PMID: 23924760

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153669 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin G-binding protein A Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 6.65 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P38507 (Residues: 213-269; Coverage: 12%)
Gene name: spa
Sequence domains: B domain
Structure domains: Immunoglobulin FC, subunit C
ZpA963 Chain: B
Molecule details ›
Chain: B
Length: 58 amino acids
Theoretical weight: 6.39 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Affibody Molecules in Biotechnological and Medical Applications.
Ståhl et al. (2017)
3 more

4 mentions without citation

The neonatal Fc receptor, FcRn, as a target for drug delivery and therapy.
Sockolosky et al. (2015)
3 more