› 2m8d

Solution NMR

Structure of SRSF1 RRM2 in complex with the RNA 5'-UGAAGGAC-3'

Released:
DOI: 10.2210/pdb2m8d/pdb
PDB entry 2m8d coloured by chain, ensemble of 16 models, front view.
PDB entry 2m8d coloured by chain, ensemble of 16 models, side view.
PDB entry 2m8d coloured by chain, ensemble of 16 models, top view.
Source organism: Homo sapiens
Primary publication:
Isolated pseudo-RNA-recognition motifs of SR proteins can regulate splicing using a noncanonical mode of RNA recognition.
Cléry A, Sinha R, Anczuków O, Corrionero A, Moursy A, Daubner GM, Valcárcel J, Krainer AR, Allain FH
Proc Natl Acad Sci U S A 110 E2802-11 (2013)
PMID: 23836656

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170575 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Serine/arginine-rich splicing factor 1 Chain: B
Molecule details ›
Chain: B
Length: 91 amino acids
Theoretical weight: 10.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07955 (Residues: 107-196; Coverage: 36%)
Gene names: ASF, OK/SW-cl.3, SF2, SF2P33, SFRS1, SRSF1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*UP*GP*AP*AP*GP*GP*AP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 8 nucleotides
Theoretical weight: 2.59 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 74%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Emerging functions of SRSF1, splicing factor and oncoprotein, in RNA metabolism and cancer.
Das et al. (2014)
11 more

13 mentions without citation

Towards understandings of serine/arginine-rich splicing factors.
Li et al. (2023)
12 more