› 2m8f

Solution NMR

Structure of lasso peptide astexin3

Released:
DOI: 10.2210/pdb2m8f/pdb
PDB entry 2m8f coloured by chain, ensemble of 20 models, front view.
PDB entry 2m8f coloured by chain, ensemble of 20 models, side view.
PDB entry 2m8f coloured by chain, ensemble of 20 models, top view.
Source organism: Asticcacaulis excentricus CB 48
Primary publication:
Discovery and characterization of an isopeptidase that linearizes lasso peptides.
Maksimov MO, Link AJ
J Am Chem Soc 135 12038-47 (2013)
PMID: 23862624

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Astexin-3 Chain: A
Molecule details ›
Chain: A
Length: 24 amino acids
Theoretical weight: 2.54 KDa
Source organism: Asticcacaulis excentricus CB 48
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: E8RUP8 (Residues: 26-49; Coverage: 49%)
Gene names: Astex_2447, AtxA3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 53%
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

New developments in RiPP discovery, enzymology and engineering.
Montalbán-López et al. (2021)
11 more

2 mentions without citation

Insights into the Unique Phosphorylation of the Lasso Peptide Paeninodin.
Zhu et al. (2016)
1 more