› 2m97

Solution NMR

Optimized Ratiometric Calcium Sensors For Functional In Vivo Imaging of Neurons and T-Lymphocytes

Released:
DOI: 10.2210/pdb2m97/pdb
PDB entry 2m97 coloured by chain, ensemble of 20 models, front view.
PDB entry 2m97 coloured by chain, ensemble of 20 models, side view.
PDB entry 2m97 coloured by chain, ensemble of 20 models, top view.
Source organism: Opsanus tau
Primary publication:
Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes.
Thestrup T, Litzlbauer J, Bartholomäus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O
Nat Methods 11 175-82 (2014)
PMID: 24390440

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Troponin C, skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 8.07 KDa
Source organism: Opsanus tau
Expression system: Escherichia coli
UniProt:
  • Canonical: W5IDB2 (Residues: 1-69; Coverage: 100%)
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 48%
Refinement method: MFR
Expression system: Escherichia coli

Quick links

Citations

61 review citations

Genetically encoded indicators of neuronal activity.
Lin et al. (2016)
60 more