› 2mbb

Solution NMR

Solution Structure of the human Polymerase iota UBM1-Ubiquitin Complex

Released:
DOI: 10.2210/pdb2mbb/pdb
PDB entry 2mbb coloured by chain, ensemble of 20 models, front view.
PDB entry 2mbb coloured by chain, ensemble of 20 models, side view.
PDB entry 2mbb coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Sparsely-sampled, high-resolution 4-D omit spectra for detection and assignment of intermolecular NOEs of protein complexes.
Wang S, Zhou P
J Biomol NMR 59 51-6 (2014)
PMID: 24789524

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139122 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase iota; Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.99 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P06654 (Residues: 229-282; Coverage: 13%)
  • Canonical: Q9UNA4 (Residues: 516-555; Coverage: 5%)
Gene names: POLI, RAD30B, spg
Sequence domains: B domain
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 78 amino acids
Theoretical weight: 8.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 86%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Applications of high dimensionality experiments to biomolecular NMR.
Nowakowski et al. (2015)
2 other articles

2 mentions without citation

Flagellum inheritance in Trypanosoma brucei requires a kinetoplastid-specific protein phosphatase.
Zhou et al. (2018)
1 more