› 2mf0

Solution NMR

Structural basis of the non-coding RNA RsmZ acting as protein sponge: Conformer L of RsmZ(1-72)/RsmE(dimer) 1to3 complex

Released:
DOI: 10.2210/pdb2mf0/pdb
PDB entry 2mf0 coloured by chain, ensemble of 20 models, front view.
PDB entry 2mf0 coloured by chain, ensemble of 20 models, side view.
PDB entry 2mf0 coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Structural basis of the non-coding RNA RsmZ acting as a protein sponge.
Duss O, Michel E, Yulikov M, Schubert M, Jeschke G, Allain FH
Nature 509 588-92 (2014)
PMID: 24828038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144064 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Translational regulator CsrA1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 70 amino acids
Theoretical weight: 7.85 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DPC3 (Residues: 1-59; Coverage: 92%)
Gene names: csrA1, rsmE
Sequence domains: Global regulator protein family
RNA_(72-MER) Chain: G
Molecule details ›
Chain: G
Length: 72 nucleotides
Theoretical weight: 23.37 KDa
Source organism: Pseudomonas fluorescens
Expression system: Not provided
Sequence domains: PrrB/RsmZ RNA family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 6%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

22 review citations

Regulation of bacterial virulence by Csr (Rsm) systems.
Vakulskas et al. (2015)
21 more

2 mentions without citation

Development of Novel In Vivo Chemical Probes to Address CNS Protein Kinase Involvement in Synaptic Dysfunction.
Watterson et al. (2013)
1 more