› 2mf8

Solution NMR

HADDOCK model of MyT1 F4F5 - DNA complex

Released:
DOI: 10.2210/pdb2mf8/pdb
PDB entry 2mf8 coloured by chain, front view.
PDB entry 2mf8 coloured by chain, side view.
PDB entry 2mf8 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A structural analysis of DNA binding by myelin transcription factor 1 double zinc fingers.
Gamsjaeger R, O'Connell MR, Cubeddu L, Shepherd NE, Lowry JA, Kwan AH, Vandevenne M, Swanton MK, Matthews JM, Mackay JP
J Biol Chem 288 35180-91 (2013)
PMID: 24097990

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115612 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Myelin transcription factor 1 Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 9.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8CFC2 (Residues: 792-880; Coverage: 8%)
Gene names: Kiaa0835, Myt1, Nzf2
Sequence domains: Zinc finger, C2HC type
DNA (5'-D(*AP*CP*CP*GP*AP*AP*AP*GP*TP*TP*CP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(*GP*TP*GP*AP*AP*CP*TP*TP*TP*CP*GP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 16%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Transcription Factors with Targeting Potential in Gliomas.
Giannopoulou et al. (2022)
8 other articles

2 mentions without citation

MYT1L mutations cause intellectual disability and variable obesity by dysregulating gene expression and development of the neuroendocrine hypothalamus.
Blanchet et al. (2017)
1 more