› 2mfg

Solution NMR

Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL4)/RsmE(dimer) 2:1 complex

Released:
DOI: 10.2210/pdb2mfg/pdb
PDB entry 2mfg coloured by chain, ensemble of 20 models, front view.
PDB entry 2mfg coloured by chain, ensemble of 20 models, side view.
PDB entry 2mfg coloured by chain, ensemble of 20 models, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Molecular basis for the wide range of affinity found in Csr/Rsm protein-RNA recognition.
Duss O, Michel E, Diarra dit Konté N, Schubert M, Allain FH
Nucleic Acids Res 42 5332-46 (2014)
PMID: 24561806

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144062 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Translational regulator CsrA1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 70 amino acids
Theoretical weight: 7.85 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DPC3 (Residues: 1-59; Coverage: 92%)
Gene names: csrA1, rsmE
Sequence domains: Global regulator protein family
SL4(RsmZ) RNA Chains: B, D
Molecule details ›
Chains: B, D
Length: 21 nucleotides
Theoretical weight: 6.77 KDa
Source organism: Pseudomonas fluorescens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 40%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Regulation of bacterial virulence by Csr (Rsm) systems.
Vakulskas et al. (2015)
11 more

2 mentions without citation

Molecular basis for the wide range of affinity found in Csr/Rsm protein-RNA recognition.
Duss et al. (2014)
1 more