› 2mgz

Solution NMR

Solution structure of RBFOX family ASD-1 RRM and SUP-12 RRM in ternary complex with RNA

Released:
DOI: 10.2210/pdb2mgz/pdb
PDB entry 2mgz coloured by chain, ensemble of 20 models, front view.
PDB entry 2mgz coloured by chain, ensemble of 20 models, side view.
PDB entry 2mgz coloured by chain, ensemble of 20 models, top view.
Source organism: Caenorhabditis elegans
Primary publication:
RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing.
Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Güntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y
Nat Struct Mol Biol 21 778-86 (2014)
PMID: 25132178

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124512 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
RRM domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.59 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: G5EEW7 (Residues: 97-189; Coverage: 23%)
Gene names: CELE_R74.5, R74.5, asd-1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RRM domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 105 amino acids
Theoretical weight: 11.61 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: O45189 (Residues: 20-123; Coverage: 42%)
Gene names: CELE_T22B2.4, T22B2.4, sup-12
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*UP*GP*CP*AP*UP*GP*GP*UP*GP*UP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.85 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 72%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

3 review citations

NMR solution structure determination of large RNA-protein complexes.
Yadav et al. (2016)
2 more

2 mentions without citation

Evidence for cooperative tandem binding of hnRNP C RRMs in mRNA processing.
Cieniková et al. (2015)
1 more