› 2mi0

Solution NMR

NMR structure of the I-V kissing-loop interaction of the Neurospora VS ribozyme

Released:
DOI: 10.2210/pdb2mi0/pdb
PDB entry 2mi0 coloured by chain, ensemble of 21 models, front view.
PDB entry 2mi0 coloured by chain, ensemble of 21 models, side view.
PDB entry 2mi0 coloured by chain, ensemble of 21 models, top view.
Source organism: Neurospora
Primary publication:
Structural insights into substrate recognition by the Neurospora Varkud satellite ribozyme: importance of U-turns at the kissing-loop junction.
Bouchard P, Legault P
Biochemistry 53 258-69 (2014)
PMID: 24325625

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196500 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3' Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 7.05 KDa
Source organism: Neurospora
Expression system: Not provided
5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.72 KDa
Source organism: Neurospora
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 54%
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

2 review citations

Chemistry and Biology of Self-Cleaving Ribozymes.
Jimenez et al. (2015)
1 more

8 mentions without citation

Insights into RNA structure and dynamics from recent NMR and X-ray studies of the Neurospora Varkud satellite ribozyme.
Dagenais et al. (2017)
7 more