› 2mkp

Solution NMR

N domain of cardiac troponin C bound to the switch fragment of fast skeletal troponin I at pH 6

Released:
DOI: 10.2210/pdb2mkp/pdb
PDB entry 2mkp coloured by chain, ensemble of 10 models, front view.
PDB entry 2mkp coloured by chain, ensemble of 10 models, side view.
PDB entry 2mkp coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Conformation of the critical pH sensitive region of troponin depends upon a single residue in troponin I.
Robertson IM, Pineda-Sanabria SE, Holmes PC, Sykes BD
Arch Biochem Biophys 552-553 40-9 (2014)
PMID: 24333682

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155812 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Troponin C, slow skeletal and cardiac muscles Chain: C
Molecule details ›
Chain: C
Length: 89 amino acids
Theoretical weight: 10.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 1-89; Coverage: 55%)
Gene names: TNNC, TNNC1
Sequence domains: EF-hand domain pair
Troponin I, fast skeletal muscle Chain: I
Molecule details ›
Chain: I
Length: 17 amino acids
Theoretical weight: 1.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48788 (Residues: 116-132; Coverage: 9%)
Gene name: TNNI2

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 38%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure and function of cardiac troponin C (TNNC1): Implications for heart failure, cardiomyopathies, and troponin modulating drugs.
Li et al. (2015)
1 other article

3 mentions without citation

Two structural motifs within canonical EF-hand calcium-binding domains identify five different classes of calcium buffers and sensors.
Denessiouk et al. (2014)
2 more