› 2mle

Solution NMR

NMR structure of the C-domain of troponin C bound to the anchoring region of troponin I

Released:
DOI: 10.2210/pdb2mle/pdb
PDB entry 2mle coloured by chain, ensemble of 20 models, front view.
PDB entry 2mle coloured by chain, ensemble of 20 models, side view.
PDB entry 2mle coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
The dilated cardiomyopathy G159D mutation in cardiac troponin C weakens the anchoring interaction with troponin I.
Baryshnikova OK, Robertson IM, Mercier P, Sykes BD
Biochemistry 47 10950-60 (2008)
PMID: 18803402

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Troponin C, slow skeletal and cardiac muscles Chain: C
Molecule details ›
Chain: C
Length: 72 amino acids
Theoretical weight: 8.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63316 (Residues: 91-161; Coverage: 44%)
Gene names: TNNC, TNNC1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 73%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Troponin structure and function: a view of recent progress.
Marston et al. (2020)
4 more