› 2mme

Hybrid

Hybrid structure of the Shigella flexneri MxiH Type three secretion system needle

Released:
DOI: 10.2210/pdb2mme/pdb
PDB entry 2mme coloured by chain, ensemble of 10 models, front view.
PDB entry 2mme coloured by chain, ensemble of 10 models, side view.
PDB entry 2mme coloured by chain, ensemble of 10 models, top view.
Source organism: Shigella flexneri
Primary publication:
High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.
Demers JP, Habenstein B, Loquet A, Kumar Vasa S, Giller K, Becker S, Baker D, Lange A, Sgourakis NG
Nat Commun 5 4976 (2014)
PMID: 25264107
Related structures: EMD-5352

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 29-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MxiH Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c
Length: 85 amino acids
Theoretical weight: 9.4 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6XVY0 (Residues: 1-83; Coverage: 100%)
Gene name: mxiH
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Experimental Method: Electron Microscopy, Solid-state NMR
Resolution: 7.7Å
Relevant EMDB volumes: EMD-5352
Expression system: Escherichia coli BL21(DE3)

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Citations

22 review citations

Bacterial Secretion Systems: An Overview.
Green et al. (2016)
21 more

4 mentions without citation

High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.
Demers et al. (2014)
3 more