› 2mnr

X-ray diffraction
1.9Å resolution

MECHANISM OF THE REACTION CATALYZED BY MANDELATE RACEMASE. 2. CRYSTAL STRUCTURE OF MANDELATE RACEMASE AT 2.5 ANGSTROMS RESOLUTION: IDENTIFICATION OF THE ACTIVE SITE AND POSSIBLE CATALYTIC RESIDUES

Released:
DOI: 10.2210/pdb2mnr/pdb
PDB entry 2mnr coloured by chain, front view.
PDB entry 2mnr coloured by chain, side view.
PDB entry 2mnr coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Mechanism of the reaction catalyzed by mandelate racemase. 2. Crystal structure of mandelate racemase at 2.5-A resolution: identification of the active site and possible catalytic residues.
Neidhart DJ, Howell PL, Petsko GA, Powers VM, Li RS, Kenyon GL, Gerlt JA
Biochemistry 30 9264-73 (1991)
PMID: 1892834

Function and Biology Details

Reaction catalysed: 
(S)-mandelate = (R)-mandelate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 38.38 KDa
Source organism: Pseudomonas putida
Expression system: Not provided
UniProt:
  • Canonical: P11444 (Residues: 3-359; Coverage: 99%)
Gene name: mdlA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I422
Unit cell:
a: 125Å b: 125Å c: 106.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.162 not available
Expression system: Not provided

Quick links

Citations

5 review citations

Divergent evolution in the enolase superfamily: the interplay of mechanism and specificity.
Gerlt et al. (2005)
4 more

16 mentions without citation

Tools for integrated sequence-structure analysis with UCSF Chimera.
Meng et al. (2006)
15 more