› 2mqv

Solution NMR

Solution NMR structure of the U5-primer binding site (U5-PBS) domain of murine leukemia virus RNA genome bound to the retroviral nucleocapsid protein

Released:
DOI: 10.2210/pdb2mqv/pdb
PDB entry 2mqv coloured by chain, ensemble of 10 models, front view.
PDB entry 2mqv coloured by chain, ensemble of 10 models, side view.
PDB entry 2mqv coloured by chain, ensemble of 10 models, top view.
Source organism: Murine leukemia virus
Primary publication:
A structure-based mechanism for tRNA and retroviral RNA remodelling during primer annealing.
Miller SB, Yildiz FZ, Lo JA, Wang B, D'Souza VM
Nature 515 591-5 (2014)
PMID: 25209668

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136828 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleocapsid protein p10-Pol Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.38 KDa
Source organism: Murine leukemia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03355 (Residues: 479-534; Coverage: 3%)
Gene name: gag-pol
Sequence domains: Zinc knuckle
RNA (68-MER) Chain: B
Molecule details ›
Chain: B
Length: 68 nucleotides
Theoretical weight: 21.89 KDa
Source organism: Murine leukemia virus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 12%
Refinement method: distance geometry
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

15 review citations

Emerging roles of tRNA in adaptive translation, signalling dynamics and disease.
Kirchner et al. (2015)
14 more