› 2mse

Solution NMR

NMR data-driven model of GTPase KRas-GNP:ARafRBD complex tethered to a lipid-bilayer nanodisc

Released:
DOI: 10.2210/pdb2mse/pdb
PDB entry 2mse coloured by chain, ensemble of 10 models, front view.
PDB entry 2mse coloured by chain, ensemble of 10 models, side view.
PDB entry 2mse coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Oncogenic and RASopathy-associated K-RAS mutations relieve membrane-dependent occlusion of the effector-binding site.
Mazhab-Jafari MT, Marshall CB, Smith MJ, Gasmi-Seabrook GM, Stathopulos PB, Inagaki F, Kay LE, Neel BG, Ikura M
Proc Natl Acad Sci U S A 112 6625-30 (2015)
PMID: 25941399

Function and Biology Details

Reactions catalysed: 
GTP + H(2)O = GDP + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-236927 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Truncated apolipoprotein A-I Chains: A, C
Molecule details ›
Chains: A, C
Length: 200 amino acids
Theoretical weight: 23.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02647 (Residues: 68-265; Coverage: 80%)
Gene name: APOA1
Sequence domains: Apolipoprotein A1/A4/E domain
GTPase KRas Chain: B
Molecule details ›
Chain: B
Length: 187 amino acids
Theoretical weight: 21.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-186; Coverage: 98%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Serine/threonine-protein kinase A-Raf Chain: D
Molecule details ›
Chain: D
Length: 73 amino acids
Theoretical weight: 8.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10398 (Residues: 19-91; Coverage: 12%)
Gene names: ARAF, ARAF1, PKS, PKS2
Sequence domains: Raf-like Ras-binding domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

4 bound ligands:
Ligand PCW 64 x PCW
Ligand 17F 16 x 17F
Ligand GNP 1 x GNP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 0%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

37 review citations

RAS Proteins and Their Regulators in Human Disease.
Simanshu et al. (2017)
36 more

7 mentions without citation

Solution NMR: A powerful tool for structural and functional studies of membrane proteins in reconstituted environments.
Puthenveetil et al. (2019)
6 more