› 2mta

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF A TERNARY ELECTRON TRANSFER COMPLEX BETWEEN METHYLAMINE DEHYDROGENASE, AMICYANIN AND A C-TYPE CYTOCHROME

Released:
DOI: 10.2210/pdb2mta/pdb
PDB entry 2mta coloured by chain, front view.
PDB entry 2mta coloured by chain, side view.
PDB entry 2mta coloured by chain, top view.
Source organism: Paracoccus denitrificans
Primary publication:
Structure of an electron transfer complex: methylamine dehydrogenase, amicyanin, and cytochrome c551i.
Chen L, Durley RC, Mathews FS, Davidson VL
Science 264 86-90 (1994)
PMID: 8140419

Function and Biology Details

Reaction catalysed: 
Methylamine + H(2)O + 2 oxidized [amicyanin] = formaldehyde + NH(3) + 2 reduced [amicyanin]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-149614 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Methylamine dehydrogenase heavy chain Chain: H
Molecule details ›
Chain: H
Length: 373 amino acids
Theoretical weight: 41.06 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P29894 (Residues: 45-417; Coverage: 96%)
Gene name: mauB
Sequence domains: Methylamine dehydrogenase heavy chain (MADH)
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Methylamine dehydrogenase light chain Chain: L
Molecule details ›
Chain: L
Length: 125 amino acids
Theoretical weight: 13.73 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P22619 (Residues: 64-188; Coverage: 95%)
Gene name: mauA
Sequence domains: Methylamine dehydrogenase, L chain
Structure domains: Methylamine/Aralkylamine dehydrogenase light chain
Amicyanin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.51 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Cytochrome c-L Chain: C
Molecule details ›
Chain: C
Length: 147 amino acids
Theoretical weight: 16.27 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P29899 (Residues: 23-169; Coverage: 95%)
Gene name: moxG
Structure domains: Cytochrome c-like domain

Ligands and Environments


Cofactor: Ligand HEC 1 x HEC
2 bound ligands:
Ligand PO4 1 x PO4
Ligand CU 1 x CU
1 modified residue:
Ligand TRQ 1 x TRQ

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 148.81Å b: 68.85Å c: 187.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 not available
Expression system: Not provided

Quick links

Citations

18 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
17 more

27 mentions without citation

Cupredoxins--a study of how proteins may evolve to use metals for bioenergetic processes.
Choi et al. (2011)
26 more