› 2mux

Solution NMR

SUMO2 non-covalently interacts with USP25 and downregulates its activity

Released:
DOI: 10.2210/pdb2mux/pdb
PDB entry 2mux coloured by chain, ensemble of 15 models, front view.
PDB entry 2mux coloured by chain, ensemble of 15 models, side view.
PDB entry 2mux coloured by chain, ensemble of 15 models, top view.
Source organism: Homo sapiens
Entry authors: Shi L, Zhang N, Wen Y

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 25 Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHP3 (Residues: 1-146; Coverage: 14%)
Gene names: USP21, USP25
Sequence domains: Ubiquitin interaction motif

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Expression system: Escherichia coli

Quick links