› 2mzn

Solution NMR

NMR structure of the HLTF HIRAN domain in its DNA-bound conformation

Released:
DOI: 10.2210/pdb2mzn/pdb
PDB entry 2mzn coloured by chain, ensemble of 20 models, front view.
PDB entry 2mzn coloured by chain, ensemble of 20 models, side view.
PDB entry 2mzn coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal.
Kile AC, Chavez DA, Bacal J, Eldirany S, Korzhnev DM, Bezsonova I, Eichman BF, Cimprich KA
Mol Cell 58 1090-100 (2015)
PMID: 26051180

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helicase-like transcription factor Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 13.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14527 (Residues: 51-171; Coverage: 12%)
Gene names: HIP116A, HLTF, RNF80, SMARCA3, SNF2L3, ZBU1
Sequence domains: HIRAN domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Expression system: Escherichia coli

Quick links

Citations

48 review citations

DNA double-strand break repair-pathway choice in somatic mammalian cells.
Scully et al. (2019)
47 more

1 mention without citation

HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal.
Kile et al. (2015)