› 2n1t

Solution NMR

Dynamic binding mode of a synaptotagmin-1-SNARE complex in solution

Released:
DOI: 10.2210/pdb2n1t/pdb
PDB entry 2n1t coloured by chain, ensemble of 5 models, front view.
PDB entry 2n1t coloured by chain, ensemble of 5 models, side view.
PDB entry 2n1t coloured by chain, ensemble of 5 models, top view.
Source organisms:
Primary publication:
Dynamic binding mode of a Synaptotagmin-1-SNARE complex in solution.
Brewer KD, Bacaj T, Cavalli A, Camilloni C, Swarbrick JD, Liu J, Zhou A, Zhou P, Barlow N, Xu J, Seven AB, Prinslow EA, Voleti R, Häussinger D, Bonvin AM, Tomchick DR, Vendruscolo M, Graham B, Südhof TC, Rizo J
Nat Struct Mol Biol 22 555-64 (2015)
PMID: 26030874

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-149303 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-associated membrane protein 2 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 8.05 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 25-93; Coverage: 60%)
Gene names: Syb2, Vamp2
Sequence domains: Synaptobrevin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 72 amino acids
Theoretical weight: 8.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 188-259; Coverage: 25%)
Gene names: Sap, Stx1a
Sequence domains: SNARE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: C
Molecule details ›
Chain: C
Length: 77 amino acids
Theoretical weight: 9.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60880 (Residues: 7-83; Coverage: 37%)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: D
Molecule details ›
Chain: D
Length: 74 amino acids
Theoretical weight: 8.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60880 (Residues: 131-204; Coverage: 36%)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptotagmin-1 Chain: E
Molecule details ›
Chain: E
Length: 156 amino acids
Theoretical weight: 17.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21579 (Residues: 272-419; Coverage: 35%)
Gene names: SVP65, SYT, SYT1
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 13%
Refinement method: molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Should I stop or should I go? The role of complexin in neurotransmitter release.
Trimbuch et al. (2016)
21 more

8 mentions without citation

Chaperoning SNARE assembly and disassembly.
Baker et al. (2016)
7 more