› 2n2k

Solution NMR

Ensemble structure of the closed state of Lys63-linked diubiquitin in the absence of a ligand

Released:
DOI: 10.2210/pdb2n2k/pdb
PDB entry 2n2k coloured by chain, ensemble of 70 models, front view.
PDB entry 2n2k coloured by chain, ensemble of 70 models, side view.
PDB entry 2n2k coloured by chain, ensemble of 70 models, top view.
Source organism: Homo sapiens
Primary publication:
Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.
Liu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C
Elife 4 (2015)
PMID: 26090905

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143328 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 71 amino acids
Theoretical weight: 8.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-679; Coverage: 10%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:
Ligand MTN 2 x MTN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 15%
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli

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Citations

7 review citations

The increasing complexity of the ubiquitin code.
Yau et al. (2016)
6 more

16 mentions without citation

An analogue-sensitive approach identifies basal body rotation and flagellum attachment zone elongation as key functions of PLK in Trypanosoma brucei.
Lozano-Núñez et al. (2013)
15 more