› 2n32

Solution NMR

NMR solution structure of the N-terminal domain of NisI, a lipoprotein from Lactococcus lactis which confers immunity against nisin

Released:
DOI: 10.2210/pdb2n32/pdb
PDB entry 2n32 coloured by chain, ensemble of 20 models, front view.
PDB entry 2n32 coloured by chain, ensemble of 20 models, side view.
PDB entry 2n32 coloured by chain, ensemble of 20 models, top view.
Source organism: Lactococcus lactis subsp. lactis
Primary publication:
The Solution Structure of the Lantibiotic Immunity Protein NisI and Its Interactions with Nisin.
Hacker C, Christ NA, Duchardt-Ferner E, Korn S, Göbl C, Berninger L, Düsterhus S, Hellmich UA, Madl T, Kötter P, Entian KD, Wöhnert J
J Biol Chem 290 28869-86 (2015)
PMID: 26459561

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nisin immunity protein Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.69 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42708 (Residues: 21-129; Coverage: 48%)
Gene name: nisI

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 93%
Refinement method: torsion angle dynamics, water refinement
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Current Knowledge of the Mode of Action and Immunity Mechanisms of LAB-Bacteriocins.
Pérez-Ramos et al. (2021)
3 more

4 mentions without citation

Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes.
Repka et al. (2017)
3 more