› 2n3e

Solution NMR

Amino-terminal domain of Latrodectus hesperus MaSp1 with neutralized acidic cluster

Released:
DOI: 10.2210/pdb2n3e/pdb
PDB entry 2n3e coloured by chain, ensemble of 20 models, front view.
PDB entry 2n3e coloured by chain, ensemble of 20 models, side view.
PDB entry 2n3e coloured by chain, ensemble of 20 models, top view.
Source organism: Latrodectus hesperus
Entry authors: Schaal D, Bauer J, Schweimer K, Scheibel T, Roesch P, Schwarzinger S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major ampullate spidroin 1 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 14.01 KDa
Source organism: Latrodectus hesperus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6YIY1 (Residues: 25-157; Coverage: 4%)
Gene name: MaSp1
Sequence domains: Major ampullate spidroin 1, spider silk protein 1, N-term
Structure domains: Enzyme I; Chain A, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 77%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

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