› 2nad

X-ray diffraction
2.05Å resolution

HIGH RESOLUTION STRUCTURES OF HOLO AND APO FORMATE DEHYDROGENASE

Released:
DOI: 10.2210/pdb2nad/pdb
PDB entry 2nad coloured by chain, front view.
PDB entry 2nad coloured by chain, side view.
PDB entry 2nad coloured by chain, top view.
Source organism: Pseudomonas sp. 101
Primary publication:
High resolution structures of holo and apo formate dehydrogenase.
Lamzin VS, Dauter Z, Popov VO, Harutyunyan EH, Wilson KS
J Mol Biol 236 759-85 (1994)
PMID: 8114093

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 43.29 KDa
Source organism: Pseudomonas sp. 101
Expression system: Not provided
UniProt:
  • Canonical: P33160 (Residues: 2-394; Coverage: 98%)
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand AZI 2 x AZI
Ligand SO4 1 x SO4
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 115.97Å b: 113.29Å c: 63.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.114 not available not available
Expression system: Not provided

Quick links

Citations

10 review citations

NAD(+)-dependent formate dehydrogenase.
Popov et al. (1994)
9 more

18 mentions without citation

Role of dynamics in enzyme catalysis: substantial versus semantic controversies.
Kohen A. (2015)
17 more