› 2nlo

X-ray diffraction
1.64Å resolution

Crystal Structure of the Quinate Dehydrogenase from Corynebacterium glutamicum

Released:
DOI: 10.2210/pdb2nlo/pdb
PDB entry 2nlo coloured by chain, front view.
PDB entry 2nlo coloured by chain, side view.
PDB entry 2nlo coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 13032
Primary publication:
1.6 angstroms structure of an NAD+-dependent quinate dehydrogenase from Corynebacterium glutamicum.
Schoepe J, Niefind K, Schomburg D
Acta Crystallogr D Biol Crystallogr D64 803-9 (2008)
PMID: 18566515

Function and Biology Details

Reaction catalysed: 
Quinate + NAD(+) = 3-dehydroquinate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinate/shikimate dehydrogenase (NAD(+)) Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 31.91 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X5C9 (Residues: 1-283; Coverage: 100%)
Gene names: aroE, qsuD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 118.784Å b: 63.021Å c: 35.527Å
α: 90° β: 92.24° γ: 90°
R-values:
R R work R free
0.149 0.148 0.165
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The shikimate dehydrogenase family: functional diversity within a conserved structural and mechanistic framework.
Peek et al. (2015)
2 other articles