› 2nnw

X-ray diffraction
2.7Å resolution

Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs

Released:
DOI: 10.2210/pdb2nnw/pdb
PDB entry 2nnw coloured by chain, front view.
PDB entry 2nnw coloured by chain, side view.
PDB entry 2nnw coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Alternative conformations of the archaeal Nop56/58-fibrillarin complex imply flexibility in box C/D RNPs.
Oruganti S, Zhang Y, Li H, Robinson H, Terns MP, Terns RM, Yang W, Li H
J Mol Biol 371 1141-50 (2007)
PMID: 17617422

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186498 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nop domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 376 amino acids
Theoretical weight: 43.52 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4M1 (Residues: 1-366; Coverage: 91%)
Gene name: PF0060
Sequence domains:
Structure domains:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase Chains: B, D
Molecule details ›
Chains: B, D
Length: 234 amino acids
Theoretical weight: 26.76 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4M2 (Residues: 1-227; Coverage: 100%)
Gene names: PF0059, flpA
Sequence domains: Fibrillarin
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3112
Unit cell:
a: 120.712Å b: 120.712Å c: 297.741Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.234 0.265
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Fibrillarin from Archaea to human.
Rodriguez-Corona et al. (2015)
5 more

8 mentions without citation

The small subunit processome in ribosome biogenesis—progress and prospects.
Phipps et al. (2011)
7 more