› 2nom

X-ray diffraction
2.4Å resolution

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound dUTP

Released:
DOI: 10.2210/pdb2nom/pdb
PDB entry 2nom coloured by chain, front view.
PDB entry 2nom coloured by chain, side view.
PDB entry 2nom coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
UTP-bound and Apo structures of a minimal RNA uridylyltransferase.
Stagno J, Aphasizheva I, Rosengarth A, Luecke H, Aphasizhev R
J Mol Biol 366 882-99 (2007)
PMID: 17189640

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.88 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q381M1 (Residues: 1-333; Coverage: 100%)
Gene names: TUT4, Tb11.01.7300
Sequence domains: Cid1 family poly A polymerase

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand DUT 2 x DUT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 79.142Å b: 41.659Å c: 104.286Å
α: 90° β: 96.46° γ: 90°
R-values:
R R work R free
0.234 0.23 0.301
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
11 more

3 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
2 more