› 2nov

X-ray diffraction
2.67Å resolution

Breakage-reunion domain of S.pneumoniae topo IV: crystal structure of a gram-positive quinolone target

Released:
DOI: 10.2210/pdb2nov/pdb
PDB entry 2nov coloured by chain, front view.
PDB entry 2nov coloured by chain, side view.
PDB entry 2nov coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Breakage-reunion domain of Streptococcus pneumoniae topoisomerase IV: crystal structure of a gram-positive quinolone target.
Laponogov I, Veselkov DA, Sohi MK, Pan XS, Achari A, Yang C, Ferrara JD, Fisher LM, Sanderson MR
PLoS One 2 e301 (2007)
PMID: 17375187

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase (ATP-hydrolyzing) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 496 amino acids
Theoretical weight: 56.46 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3HYJ3 (Residues: 1-487; Coverage: 59%)
Gene name: parC
Sequence domains: DNA gyrase/topoisomerase IV, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I222
Unit cell:
a: 136.92Å b: 137.85Å c: 326.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.276
Expression system: Escherichia coli

Quick links

Citations

4 review citations

DNA topoisomerases: harnessing and constraining energy to govern chromosome topology.
Schoeffler et al. (2008)
3 more

6 mentions without citation

Probing designability via a generalized model of helical bundle geometry.
Grigoryan et al. (2011)
5 more