› 2np2

X-ray diffraction
3.02Å resolution

Hbb-DNA complex

Released:
DOI: 10.2210/pdb2np2/pdb
PDB entry 2np2 coloured by chain, front view.
PDB entry 2np2 coloured by chain, side view.
PDB entry 2np2 coloured by chain, top view.
Source organism: Borreliella burgdorferi
Primary publication:
Shaping the Borrelia burgdorferi genome: crystal structure and binding properties of the DNA-bending protein Hbb.
Mouw KW, Rice PA
Mol Microbiol 63 1319-30 (2007)
PMID: 17244195

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115675 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA-binding protein HU Chains: A, B
Molecule details ›
Chains: A, B
Length: 108 amino acids
Theoretical weight: 12.69 KDa
Source organism: Borreliella burgdorferi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57267 (Residues: 1-108; Coverage: 100%)
Gene names: BB_0232, hbb, hup
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
36-MER Chains: C, D
Molecule details ›
Chains: C, D
Length: 36 nucleotides
Theoretical weight: 11.06 KDa
Source organism: Borreliella burgdorferi
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 59.27Å b: 88.32Å c: 96.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Gene regulation in Borrelia burgdorferi.
Samuels DS. (2011)
2 more

9 mentions without citation

Analyzing and building nucleic acid structures with 3DNA.
Colasanti et al. (2013)
8 more