› 2np5

X-ray diffraction
1.8Å resolution

Crystal structure of a transcriptional regulator (RHA1_ro04179) from Rhodococcus sp. Rha1.

Released:
DOI: 10.2210/pdb2np5/pdb
PDB entry 2np5 coloured by chain, front view.
PDB entry 2np5 coloured by chain, side view.
PDB entry 2np5 coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Chruszcz M, Evdokimova E, Kagan O, Cymborowski M, Savchenko A, Edwards A, Joachimiak A, Minor W, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-171129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 203 amino acids
Theoretical weight: 21.83 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0S914 (Residues: 1-203; Coverage: 100%)
Gene name: RHA1_ro04179
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
Ligand LMT 2 x LMT
Ligand NDS 3 x NDS
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P1
Unit cell:
a: 50.721Å b: 52.23Å c: 72.853Å
α: 102.31° β: 107.25° γ: 89.71°
R-values:
R R work R free
0.179 0.177 0.219
Expression system: Escherichia coli

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