› 2nqp

X-ray diffraction
3.5Å resolution

Crystal structure of pseudoudirinde synthase TruA in complex with leucyl tRNA

Released:
DOI: 10.2210/pdb2nqp/pdb
PDB entry 2nqp coloured by chain, front view.
PDB entry 2nqp coloured by chain, side view.
PDB entry 2nqp coloured by chain, top view.
Source organisms:
Primary publication:
How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA.
Hur S, Stroud RM
Mol Cell 26 189-203 (2007)
PMID: 17466622

Function and Biology Details

Reaction catalysed: 
tRNA uridine(38-40) = tRNA pseudouridine(38-40)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-139676 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA pseudouridine synthase A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 270 amino acids
Theoretical weight: 30.44 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07649 (Residues: 1-270; Coverage: 100%)
Gene names: JW2315, asuC, b2318, hisT, leuK, truA
Sequence domains: tRNA pseudouridine synthase
Structure domains:
transfer RNA Chain: F
Molecule details ›
Chain: F
Length: 87 nucleotides
Theoretical weight: 28.08 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
Ligand K 6 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 96.278Å b: 128.585Å c: 159.256Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.246 0.279
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

Pseudouridine: still mysterious, but never a fake (uridine)!
Spenkuch et al. (2014)
11 more

4 mentions without citation

Pseudouridines in RNAs: switching atoms means shifting paradigms.
Lin et al. (2021)
3 more