› 2nr4

X-ray diffraction
1.85Å resolution

Crystal structure of FMN-bound protein MM1853 from Methanosarcina mazei, Pfam DUF447

Released:
DOI: 10.2210/pdb2nr4/pdb
PDB entry 2nr4 coloured by chain, front view.
PDB entry 2nr4 coloured by chain, side view.
PDB entry 2nr4 coloured by chain, top view.
Source organism: Methanosarcina mazei
Entry authors: Bonanno JB, Gilmore J, Bain KT, Lau C, Wasserman S, Smith D, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185811 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conserved protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 213 amino acids
Theoretical weight: 23.96 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PVV4 (Residues: 2-212; Coverage: 100%)
Gene name: MM_1853
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P63
Unit cell:
a: 139.334Å b: 139.334Å c: 55.391Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.256
Expression system: Escherichia coli BL21(DE3)

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