› 2nr9

X-ray diffraction
2.2Å resolution

Crystal structure of GlpG, Rhomboid Peptidase from Haemophilus influenzae

Released:
DOI: 10.2210/pdb2nr9/pdb
PDB entry 2nr9 coloured by chain, front view.
PDB entry 2nr9 coloured by chain, side view.
PDB entry 2nr9 coloured by chain, top view.
Source organism: Haemophilus influenzae 86-028NP
Primary publication:
The crystal structure of the rhomboid peptidase from Haemophilus influenzae provides insight into intramembrane proteolysis.
Lemieux MJ, Fischer SJ, Cherney MM, Bateman KS, James MN
Proc Natl Acad Sci U S A 104 750-4 (2007)
PMID: 17210913

Function and Biology Details

Reaction catalysed: 
Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-155206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhomboid protease GlpG Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.14 KDa
Source organism: Haemophilus influenzae 86-028NP
Expression system: Escherichia coli
UniProt:
  • Canonical: P44783 (Residues: 1-192; Coverage: 100%)
Gene names: HI_0618, glpG
Sequence domains: Rhomboid family
Structure domains: Rhomboid-like

Ligands and Environments

2 bound ligands:
Ligand PA6 3 x PA6
Ligand PQE 2 x PQE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 121.288Å b: 35.047Å c: 52.931Å
α: 90° β: 104.02° γ: 90°
R-values:
R R work R free
0.236 0.233 0.295
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Alzheimer's disease: from pathology to therapeutic approaches.
Jakob-Roetne et al. (2009)
38 more

19 mentions without citation

Taking the plunge: integrating structural, enzymatic and computational insights into a unified model for membrane-immersed rhomboid proteolysis.
Urban S. (2010)
18 more