› 2nrt

X-ray diffraction
1.5Å resolution

Crystal structure of the C-terminal half of UvrC

Released:
DOI: 10.2210/pdb2nrt/pdb
PDB entry 2nrt coloured by chain, front view.
PDB entry 2nrt coloured by chain, side view.
PDB entry 2nrt coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structure of the C-terminal half of UvrC reveals an RNase H endonuclease domain with an Argonaute-like catalytic triad.
Karakas E, Truglio JJ, Croteau D, Rhau B, Wang L, Van Houten B, Kisker C
EMBO J 26 613-22 (2007)
PMID: 17245438

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UvrABC system protein C Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 25.39 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYA3 (Residues: 339-557; Coverage: 39%)
Gene names: TM_0265, uvrC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 35.375Å b: 72.782Å c: 91.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.221
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
9 more

7 mentions without citation

XPB and XPD helicases in TFIIH orchestrate DNA duplex opening and damage verification to coordinate repair with transcription and cell cycle via CAK kinase.
Fuss et al. (2011)
6 more