› 2nrx

X-ray diffraction
1.9Å resolution

Crystal structure of the C-terminal half of UvrC, in the presence of sulfate molecules

Released:
DOI: 10.2210/pdb2nrx/pdb
PDB entry 2nrx coloured by chain, front view.
PDB entry 2nrx coloured by chain, side view.
PDB entry 2nrx coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structure of the C-terminal half of UvrC reveals an RNase H endonuclease domain with an Argonaute-like catalytic triad.
Karakas E, Truglio JJ, Croteau D, Rhau B, Wang L, Van Houten B, Kisker C
EMBO J 26 613-22 (2007)
PMID: 17245438

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UvrABC system protein C Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 25.39 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYA3 (Residues: 339-557; Coverage: 39%)
Gene names: TM_0265, uvrC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 35.359Å b: 81.015Å c: 99.619Å
α: 90° β: 98.02° γ: 90°
R-values:
R R work R free
0.187 0.184 0.24
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
11 more

1 mention without citation

Structure of the C-terminal half of UvrC reveals an RNase H endonuclease domain with an Argonaute-like catalytic triad.
Karakas et al. (2007)