› 2ns0

X-ray diffraction
2Å resolution

Crystal structure of protein RHA04536 from Rhodococcus sp

Released:
DOI: 10.2210/pdb2ns0/pdb
PDB entry 2ns0 coloured by chain, front view.
PDB entry 2ns0 coloured by chain, side view.
PDB entry 2ns0 coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Chang C, Skarina T, Onopriyenko O, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine tRNA ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.42 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0S2K3 (Residues: 1-85; Coverage: 100%)
Gene name: RHA1_ro06458
Sequence domains: Protein of unknown function (DUF3253)
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2221
Unit cell:
a: 37.683Å b: 53.165Å c: 90.062Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.216
Expression system: Escherichia coli

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