› 2nsl

X-ray diffraction
2Å resolution

E. coli PurE H45N mutant complexed with CAIR

Released:
DOI: 10.2210/pdb2nsl/pdb
PDB entry 2nsl coloured by chain, front view.
PDB entry 2nsl coloured by chain, side view.
PDB entry 2nsl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
N5-CAIR mutase: role of a CO2 binding site and substrate movement in catalysis.
Hoskins AA, Morar M, Kappock TJ, Mathews II, Zaugg JB, Barder TE, Peng P, Okamoto A, Ealick SE, Stubbe J
Biochemistry 46 2842-55 (2007)
PMID: 17298082

Function and Biology Details

Reaction catalysed: 
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide mutase Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 17.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG18 (Residues: 1-169; Coverage: 100%)
Gene names: JW0512, b0523, purE
Sequence domains: AIR carboxylase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand C2R 1 x C2R
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I422
Unit cell:
a: 111.317Å b: 111.317Å c: 49.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.224
Expression system: Escherichia coli

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Citations

1 review citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
15 other articles

3 mentions without citation

The Recognition of Identical Ligands by Unrelated Proteins.
Barelier et al. (2015)
2 more